Aperçu
Les kits d’extraction d’ADN « cfPure® Cell Free” sont conçus pour une purification rapide et efficace des ADN circulants libres de cellules (cfDNA). Le système utilise des particules magnétiques recouvertes de silice pour purifier ces ADN libres circulants depuis 1 ml jusqu’à plus de 10 ml de sérum ou de plasma. Les tampons ont été développé pour assurer l’efficacité de l’extraction de fragments d’ADN de 100 paires de bases à 500 paires de bases afin de maximiser la récupération d’ADN libres circulants. L’ADN récupéré convient à une large gamme d’applications en aval comme le séquençage NGS, les qPCR et les ddPCR.
Applications:
Extrait un ADN libre circulant de haute qualité à partir du plasma, sérum, et de l’urine pour le séquençage de nouvelle génération ( NGS), la PCR, la qPCR, et la ddPCR et bien d’autres applications.
Advantages and Features:
- • Versatile: Extraction from <1mL to >10 mL serum or plasma – in single tubes or 24 to 96-well plates
- • Automation Friendly: On both the KingFisher™ and liquid handling instruments, with extractions taking approximately 50 minutes
- • Time Efficient: Manual extraction protocol takes less than 75 minutes
- • Cost Effective: Competitive price <3€ per extraction from 1 mL plasma (List price for 250 mL kit)
- • Elution volumes: Range from 15 µL to 50 µL
cfDNA Kit Yields Reproducible Results
Plasma ID | Total DNA Recovered (ng) |
A | 7.00 |
A | 7.05 |
B | 32.45 |
B | 33.50 |
C | 26.80 |
C | 27.70 |
D | 10.90 |
D | 10.90 |
Efficient Recovery of cfDNA Relative to Competition
Extraction Kit | Sample ID | cfDNA recovered per ml of plasma (ng) | 115 nt ALU sequence cfDNA per ml of plasma (ng) (by PCR) |
Competitor T | 1 | 4.99 | 1.97 |
BioChain’s cfPure | 1 | 6.35 | 2.53 |
Competitor T | 2 | 6.18 | 3.21 |
BioChain’s cfPure | 2 | 8.28 | 3.93 |
Competitor T | 3 | 6.93 | 3.79 |
BioChain’s cfPure | 3 | 7.50 | 3.87 |

Figure 1. cfPure® Cell Free DNA Kit was used to isolate DNA from 5 ml of healthy human plasma. 62 ng of cfDNA (150-200 bp fragments) were recovered with low amounts of gDNA contamination.

cfDNA Validated with NGS by End Users
cfDNA extracted by cfPure® cfDNA Extraction kit is excellent for downstream applications such as NGS. In a third party study cfDNA was extracted from 10 ml’s of human plasma from 4 donors using BioChain’s cfPure® (B), Competitor T’s(T), and Competitor Q’s (Q, column-based) cfDNA extraction kits. Libraries were then constructed for each sample , and whole genome sequencing was performed . From the data analysis, three extraction methods did not show significant performance difference across the board: Insert size, Mapping rate, Whole genome coverage, Substitution rates and GC bias.


Automation Ready
cfPure® Cell Free DNA extraction kit is applicable on both the KingFisher™ and liquid handling instruments. With the KingFisher™ Flex and BioChain’s cfPure® Cell Free DNA Extraction kit can extract up to 96 one ml samples in 50 minutes, or 24 five ml samples in one hour.

Specifications
Sample Type (General): | Liquid Samples (e.g. Serum), dsDNA |
---|---|
High Throughput Compatibility: | Automated Protocols |
Downstream Application: | Next-Generation Sequencing, PCR, Real-Time PCR |
Product Line: | cfPure® |
Product Size: | 1 kit |
Shipping Condition: | Room Temperature |
Kit Contents
K5011610 | K5011625 | K5011625MA |
---|---|---|
2 x 1.33 mL cfPure Cell Free DNA Magnetic Beads | 5 x 1.33 mL cfPure Cell Free DNA Magnetic Beads | 5 x 1.33 mL cfPure Cell Free DNA Magnetic Beads |
1 x 115 mL cfPure Cell Free DNA Lysis/Binding Solution | 3 x 95 mL cfPure Cell Free DNA Lysis/Binding Solution | 1 x 95 mL cfPure Cell Free DNA Lysis/Binding Solution |
2 x 55 mL cfPure Cell Free DNA Wash Solution | 5 x 55 mL cfPure Cell Free DNA Wash Solution | 1 x 65 mL cfPure Cell Free DNA Wash Solution |
1 x 6 mL cfPure Cell Free DNA Elution Solution | 1 x 15 mL cfPure Cell Free DNA Elution Solution | 1 x 15 mL cfPure Cell Free DNA Elution Solution |
Citations
Lennon AM, Buchanan AH, Kinde I, et al.
[published online April 28, 2020]. Science. doi: 10.1126/science.abb9601
Assessing aneuploidy with repetitive element sequencing.
Douville C, Cohen JD, Ptak J, Popoli M, Schaefer J, Silliman N, Dobbyn L, Schoen RE, Tie J, Gibbs P, Goggins M, Wolfgang CL, Wang TL, Shih IM, Karchin R, Lennon AM, Hruban RH, Tomasetti C, Bettegowda C, Kinzler KW, Papadopoulos N, Vogelstein B.
Proc Natl Acad Sci U S A. 2020 Mar 3;117(9):4858-4863. doi: 10.1073/pnas.1910041117. Epub 2020 Feb 19. PMID: 32075918
Cell-free DNA in cancer: current insights.
Fettke H, Kwan EM, Azad AA.
Cell Oncol (Dordr). 2019 Feb;42(1):13-28. doi: 10.1007/s13402-018-0413-5. Epub 2018 Oct 26. Review.PMID: 30367445